Fig. 4

Control of expression of the voltage-gated potassium channel Kv4.3 and the bi-directional membrane ion transporter NCX in the rat right ventricle. a, d mRNA levels of Kv4.3 (a) and NCX (d) by real time PCR semi-quantitative (q) analysis normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. mRNA level is given as the mean ± standard error of the mean (SEM). Asterisk Significantly different from control at p < 0.05. n = 6 rats per group. b, e Representative western blots comparing Kv4.3 (b) and NCX (e) expression in the control and finasteride-, testosterone-, and flutamide-treated right ventricular lysates. c, f Quantification of three replicate immunoblots for Kv4.3 (c) and NCX (d). Protein level is given as mean ± SEM related to the housekeeping β-tubulin (Tub). Asterisk Significantly different from control at p < 0.05. n = 5 rats per group