Fig. 3

Localization of TUNEL positive nuclei. Soleus muscle cross sections were immunoreacted with laminin (green), TUNEL-positive nuclei (red), and DAPI (blue) (a). TUNEL positive interstitial cells were observed in the soleus muscles (arrow). The scale bar in the picture is 50 μm. The number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive nuclei (per section and fiber CSA) in the soleus muscle after 7 days of hindlimb unloading (b, c). Values are mean ± SE; n = 5 per group. *p < 0.05 versus CT; †p < 0.05 versus HU