Fig. 1From: Expression of the TRPM6 in mouse placental trophoblasts; potential role in maternal–fetal calcium transportTRPM6 mRNA expression in mouse placenta: a, b quantitative PCR of TRPM6 or TRPM7 in mouse placenta at 14 or 18 day post-fertilization. The increase of TRPM6 mRNA was statistically significant (p < 0.01, n = 7, a), whereas TRPM7 mRNA level was not significantly changed during development (n = 7, b). c–h In situ hybridization of β-actin or TRPM6 in mouse placenta at 18 days post-fertilization. Labyrinthine trophoblasts were labeled by TRPM6 antisense probe (n = 5, e, g) but not by sense probe (f, h). Almost all the cells were stained by antisense probe of β-actin (c). No significant staining was observed in a negative control without DIG-labeled probes (d). Scale bars 100 μmBack to article page