Fig. 4
Estrogen induced osteoclast apoptosis by increasing RIP140 expression through the Fas/FasL pathway. a Regulation of caspase-3 activity by estrogen. Osteoclasts were treated for 12 h with 10−8 M estrogen. n = 3, **p < 0.01. b The levels of Bcl-2 and Bax protein from osteoclasts treated with estrogen were analyzed by Western blot. c Western-blot analysis of RIP140 protein expression in osteoclasts treated with 10−8 M estrogen for 12 h. d qRT-PCR analysis of RIP140 mRNA expression in osteoclasts treated with 10−8 M estrogen for 12 h. n = 3, **p < 0.01, *p < 0.05. e The effects of estrogen and Lentivirus-shRNA-targeting RIP140 on caspase-3 activity in osteoclasts. n = 3, **p < 0.01, *p < 0.05. f The levels of Bcl-2 and Bax protein from osteoclasts treated with estrogen and Lentivirus-shRNA-targeting RIP140 were analyzed by Western blot. g qRT-PCR analysis of FasL mRNA expression in osteoclasts treated with 10−8 M estrogen for 12 h. n = 3, **p < 0.01. h qRT-PCR analysis of Fas mRNA expression in osteoclasts treated with 10−8 M estrogen for 12 h. n = 3. i Western-blot analysis of RIP140 protein expression in osteoclasts treated with estrogen and Lentivirus-shRNA-targeting FasL. j qRT-PCR analysis of RIP140 mRNA expression in osteoclasts treated with estrogen and Lentivirus-shRNA-targeting FasL. n = 3, **p < 0.01, *p < 0.05. Con control, NC negative control, E2 17β-estradiol. k A schematic diagram of RIP140-involved pathways on estrogen-regulated osteoclastogenesis, osteoclast apoptosis, and bone resorption