Fig. 3
Involvement of RIP140 in estrogen-inhibited osteoclast differentiation and bone resorption. a Verified RIP140 knockdown effect by Western blot in lentivirus-mediated transduction of primary culture osteoclasts precursors. b Verified RIP140 knockdown effect by qRT-PCR in lentivirus-mediated transduction of primary culture osteoclasts precursors. n = 3, **p < 0.01. c TRAP staining of osteoclasts precursors with or without 10−8 M estrogen and transfected with lentivirus-shRNA-targeting RIP140. d Summarized data showed that the depletion of RIP140 significantly attenuated the inhibitor effect of estrogen on osteoclastogenesis. n = 3, *p < 0.05. **p < 0.01. e Osteoclast activity was measured by release of europium-labeled collagen measured by florescence. n = 3, *p < 0.05. **p < 0.01. f Pit formation assay of osteoclasts precursors with or without 10−8 M estrogen and transfection with Lentivirus-shRNA-targeting RIP140. g Summarized data showed that the depletion of RIP140 attenuated the inhibitor effect of estrogen on osteoclasts bone resorption activity. n = 3, *p < 0.05. **p < 0.01. h–j qRT-PCR analysis of osteoclasts marker gene in osteoclasts precursors with or without estrogen and transfection with Lentivirus-shRNA-targeting RIP140. Con control, NC negative control, E2 17β-estradiol