Fig. 2

The effect of estrogen on RIP140 expression during osteoclast differentiation in vivo. a Representative figures of micro-CT analysis of the distal end of intact femurs of mice in different group. Scale bar 10 µm. b BMD in the distal end of intact femurs was showed in different groups. n = 4, **p < 0.01. c BV/TV in the distal end of intact femurs was showed in different groups. n = 4, **p < 0.01. d Tr.Sp in the distal end of intact femurs was showed in different groups. n = 4, **p < 0.01. e Representative figures of hematoxylin-eosin staining of the distal end of intact femurs of mice in different group. Scale bar 25 µm. f Western-blot analysis of RIP140 protein expression during osteoclast differentiation process in a different group. g qRT-PCR analysis of RIP140 mRNA expression during osteoclasts differentiation process in a different group. n = 4, **p < 0.01. E2 17β-estradiol, BMD bone mineral density, BV/TV bone volume/tissue volume, Tr.Sp trabecular separation/spacing