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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: The analysis of antioxidant expression during muscle atrophy induced by hindlimb suspension in mice

Fig. 4

Changes in the antioxidant defense system in HLS mice after 2 weeks of treatment and CTL mice. a mRNA expression level of antioxidants determined using quantitative real-time PCR [n = 5 for the superoxide dismutases (SOD) SOD2 and SOD3, peroxiredoxins (Prdx) Prdx4 and Prdx5, glutathione peroxidases (GPX) GPX2 and GPX3, and catalase; n = 3 for the other targets]. b–d Representative immunoblots (upper, the numbers [1, 2, and 3] indicate biological replicates) and densitometric analysis (bottom) of antioxidant protein levels (n = 3 for Prdx1, Prdx4, Prdx6, and SOD3; n = 4 for SOD1, SOD2, and GPX3; n = 7 for Prdx2, Prdx3, Prdx5, GPX1, GPX2 and catalase. Values are presented as the mean ± SE. Single asterisk and double asterisk indicate a significant difference between HLS and CTL mice at p < 0.05 and p < 0.01, respectively

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