Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells

Table 1 General electrical properties of the two groups of AFSC approached via whole-cell patch-clamp

Feature	R _p (MΩ)	C _m (pF)	R _m (MΩ)	R _a (MΩ)	τ (μs)	I _hold (pA)	Preservation of the seal at the end of exp.
Senescent AFSC (n = 18)
Mean	2.03	47.27	2021.34	7.62	370.55	−179.17	72.22 %
SD	0.83	22.28	2253.96	4.44	291.41	295.54
Cryopreserved AFSC (n = 19)
Mean	2.33	44.97	1519.67	8.92	415.46	−100.95	78.94 %
SD	0.77	26.75	1927.00	4.24	330.09	95.71

R _p pipette resistance, C _m membrane capacitance, R _m membrane resistance, R _a access resistance, τ time constant of the equivalent electrical circuit, I _hold holding current at −80 mV

ISSN: 1880-6562