Fig. 5

Markers suggestive of neuronal progenitor commitment in cryopreserved AFSC. a Immunofluorescent staining (green AlexaFluor^® 488, with bright violet DAPI nuclear counterstaining) for β3-tubulin, NF-200, and a negative control (CTRL-) lacking primary antibody. b Flow cytometry immunophenotyping for β3-tubulin, NF-200, and CD56/NCAM (distributions in grey) vs. negative control isotypes (distributions in black). c RT-PCR amplification products (agarose gel electrophoresis and ethidium bromide staining) for human neuroD1, GIRK2, and control gene β-actin; last lane represents a no-template control. Right relative quantitation of expression (SYBR^® Green qPCR, ΔC _T relative to β-actin average) for the four replicates of neuroD1 and GIRK2 (individual values, mean ± SEM)