Fig. 1
From: Inhibition of monocarboxylate transporter 1 suppresses the proliferation of glioblastoma stem cells
Generation of CSCs by the sphere formation assay. a Glioblastoma cell lines (U-251 MG and U87-MG) formed spheres by the sphere formation assay (scale bars 200 μm). b Stem cell marker (Nestin, Nanog, CD133, Sox-2, and Oct-4) mRNA expression levels were increased in CSCs. 18S rRNA was used as an internal control (n = 3, mean ± SEM; *p < 0.05, **p < 0.01 vs. non-CSC). c, d Immunofluorescence analysis shows that the expression of stem cell markers was increased in spheres. Pimonidazole, a hypoxia probe, accumulated in spheres (scale bars 100 μm). CSC cancer stem cell