Fig. 6
a Representative Western-blot patterns of CD68, transforming growth factor-β (TGF-β), heat shock protein 72 (HSP72), and Ponceau-S staining in soleus muscles of Con, BPVC, Ice, and Heat groups 3, 7, 15, and 28 days after bupivacaine injection. b–d The protein expression levels of CD68 (b), TGF-β (c), and HSP72 (d) in each group during the experimental periods. The optical density (OD) values of CD68, TGF-β, and HSP72 are expressed as percentages relative to the average level in the Con group at day 3 (100 %). Values are means ± SEM. n = 6–12/group at each time point. See Fig. 1 for the abbreviations. #,§Significant main effect of treatment and time revealed by two-way ANOVA, respectively (p < 0.05). ¶Significant interaction between treatment and time revealed by two-way ANOVA (p < 0.05). *Significantly different from Con group in the same time point (p < 0.05). a,bSignificantly different from day 3 and day 7 in each group, respectively (p < 0.05)