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Fig. 5 | The Journal of Physiological Sciences

Fig. 5

From: Effects of icing or heat stress on the induction of fibrosis and/or regeneration of injured rat soleus muscle

Fig. 5

a Typical immunohistochemical staining images of Pax7 (red) and laminin (green) with nuclear staining by 4′,6-diamidino-2-phenilindole (DAPI, blue) in the cross sections of soleus muscles in all experimental groups at day 7. Arrowheads indicate the Pax7-positive (Pax7+) satellite cells. Scale bars 50 μm. b Changes in the number of Pax7+ satellite cells per fiber in Con, BPVC, Ice, and Heat groups during the experimental periods. Values are means ± SEM. n = 3–5/group at each time point. See Fig. 1 for the abbreviations. #,§Significant main effect of treatment and time revealed by two-way ANOVA, respectively (p < 0.05). ¶Significant interaction between treatment and time revealed by two-way ANOVA (p < 0.05). *Significantly different from Con in the same time point (p < 0.05). aSignificantly different from day 3 in each group (p < 0.05)

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