Fig. 3
a Typical immunohistochemical staining images of laminin (green) and desmin (red) in the cross sections of soleus muscles in BPVC, Ice, and Heat groups at day 28. Scale bars 50 μm. b Changes in the mean cross-sectional area of soleus muscle fibers in each group during the experimental period. Values are means ± SEM. n = 3/group at each time point. See Fig. 1 for the abbreviations. #,§Significant main effect of treatment and time revealed by two-way ANOVA, respectively (p < 0.05). ¶Significant interaction between treatment and time revealed by two-way ANOVA (p < 0.05). *Significantly different from Con group in the same time point (p < 0.05). b,cSignificantly different from day 7 and day 15 in each group, respectively (p < 0.05)