Fig. 5

Quantification of frontopolar cortex from MDD and healthy control. a Negative controls of flow cytometry staining with IgG. b A novel cell counting method can separate nuclei from postmortem brain into neurons (NeuN⁺) and oligodendrocytes (Olig2⁺), astrocytes, and microglia (NeuN⁻/Olig2⁻). c The Olig2⁺ nuclei were further divided into two populations of strongly and weakly Olig2-positive nuclei, which are oligodendrocyte precursor cells and oligodendrocytes, respectively. The density of Olig2⁺ (d), Olig2^weak+ (e), and Olig2^strong+ (f) nuclei in 1 mg of gray matter from the frontopolar cortex (×10³ cells/mg). *p < 0.05 by unpaired t test. Results of immunostaining with Ki-67, a marker of proliferating cells, in the hippocampus (g) and subependymal zone (h) of adult cynomolgus monkeys. Ki-67⁺ cells (brown, arrowhead) are present in both brain regions. Nuclei are counterstained with hematoxylin (purple). Scale bar 40 μm. i Proportion of Olig2^strong+ nuclei in the frontopolar cortex of healthy controls. A part of this figure was rearranged from [49]