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Fig. 5 | The Journal of Physiological Sciences

Fig. 5

From: The hyperpolarization-activated cyclic nucleotide-gated (HCN) channels contain multiple S-palmitoylation sites

Fig. 5

ΔCysNT mutation does not influence the current of HCN2. a Representative currents of HCN2 (left) and ΔCysNT mutant (right) expressed in Xenopus oocytes. Membrane potential was held at −20 mV, and was stepped from −20 to −140 mV in −10 mV increments for 2 s, and then to 0 mV to record the tail currents. b Current–voltage (I–V) relationships measured at the pulse end (n = 20). Maximum current amplitude of ΔCysNT mutant was not significantly changed (p = 0.073 versus HCN2 at −140 mV). c Activation curves obtained from tail currents. Amplitudes of the tail currents at 0 mV following step pulses were normalized to the maximum amplitude and plotted against the membrane potential during the step pulses (n = 20). The continuous lines are the Boltzmann’s equation fitted to the relationships (see “Results”)

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