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Fig. 1 | The Journal of Physiological Sciences

Fig. 1

From: Sleep as a biological problem: an overview of frontiers in sleep research

Fig. 1

The two-photon imaging of sleeping mice. a Schematic of a two-photon microscopy apparatus for naturally waking/sleeping mice. This experimental apparatus is based on the rig proposed by David Tank’s laboratory [118]. Mice can fall asleep spontaneously even under the head-restrained condition owing to a floating trackball. For detection of sleep state, EEG/EMG signals are recorded simultaneously with two-photon imaging. b Two-photon image of GCaMP-expressing neurons (green) in the layer 2/3 of primary motor cortex

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