Fig. 4

Two-PM images of the anastomotic region in an NSC-transplanted and MOS-treated YFP mouse for 2 weeks. PKH26 fluorescence (+)/YFP fluorescence (+) [PKH26 (+)/YFP (+)] neurons distributed in each of the three areas (b-1, -2, -3) of each of nine field (a-1–c-3; field size: 310 µm × 310 µm) around the knot at the anastomosis were demonstrated. Two blue lines (upper square) indicate both ends of the anastomosis. a Mid-right areas (b-3) were demonstrated at depths of 107 and 110 µm. Each white arrowhead indicated nucleus. b A mid-right area (b-3) was demonstrated three dimensionally from a direction shown by a thick white arrow. Yellow arrows indicate clustered PKH26 (+) neurons. c Mid-mid areas (b-2; knot area) were demonstrated at depths of 123 and 131 µm. d Mid-left areas (b-1) were demonstrated at depths of 121 and 134 µm. In (a, c, d), orange solid arrows indicated PKH26 (+) neurons, and yellow solid arrows indicated YFP (+) neurons (not all). Overlapped neurons between both depths were not counted, as indicated by each open arrow