Fig. 2

Age-related expression of mPer2 sl-RNA–binding proteins in the liver. a Western blotting analysis of age-related hnRNP H and hnRNP C expression using monoclonal antibodies and 10 μg of male mouse liver NE. Molecular weight is indicated on the right, and the age of the animals is shown at the top. b Graphical illustration of the patterns of hnRNP C1/C2 and hnRNP H/F expression as shown in (a). Protein expression is shown as the total mean intensity of all bands at each age. c 2DE analysis of age-related expression of hnRNP C1/C2 in the liver. Representative images of 2DE gels for 3-month-old mice. CBB stained spots are shown in the images from immobilized pH 4–7, 5–8, and 6–11 gradient IEF gels and 10–16 % gradient SDS-PAGE gels. Spots identified as hnRNP C1/C2 are shown with red circles. d Age-related expression profiles of spots identified as hnRNP C1/C2. e 2DE analysis of age-related expression of hnRNP H1/H2 in the liver. Spots identified as hnRNP H1/H2 are shown with a red circle. The location of each spot is shown with red circle in Figs. S6, S7 and S8. f Age-related expression profiles of spots identified as hnRNP H1/H2. Spot N5525 in Table 2 is not shown because the intensity of spot was very low. Mouse liver NEs were sampled between 0930 and 1200 hours (lights on between 0600 and 1800 hours) (color figure online)