Trans and interesterified fat and palm oil during the pregnancy and lactation period inhibit the central anorexigenic action of insulin in adult male rat offspring

Table 1 Diet composition (g/kg)

Constituents (g/kg of diet)	Control diet (soy oil)	Partially hydrogenated fat diet (trans)	Palm oil diet	Interesterified fat diet
Casein (vitamin free)	200.0 (140.0)	200.0	200.0	200.0
Cornstarch	397.4 (465.7)	397.4	397.4	397.4
Dextrinized cornstarch	132.0 (155)	132.0	132.0	132.0
Sucrose	100.0	100.0	100.0	100.0
Cellulose	50.0	50.0	50.0	50.0
Salt mix G^a (M)^b	10.0	10.0	10.0	10.0
Vitamin mix^c	35.0	35.0	35.0	35.0
β-Choline	2.5	2.5	2.5	2.5
Butylhydroquinone-BHT	0.014	0.01	0.01	0.01
Soy oil	70.0 (40.0)	10.0	20.0	20.0
Partially hydrogenated fat	–	60.0	–	–
Palm oil	–	–	50.0	–
Interesterified fat	–	–	–	50.0
Energy value (kcal/kg)	3,950	3,950	3,950	3,950

Amounts during maintenance periods (from 60 days of age onwards) are presented within parentheses (Reeves)
^aSalt mix G (mg/kg diet) for growth, pregnancy and lactation periods: calcium 5,000.0, phosphorus 1,561.0, potassium 3,600.0, sulphur 300.0, sodium 1,019.0, chloride 1,571.0, magnesium 507.0, iron 35.0, zinc 30.0, manganese 10.0, copper 6.0, iodine 0.2, molubdenum 0.15, selenium 0.15, silicon 5.0, chromium 1.0, fluoride 1.0, nickel 0.5, boron 0.5, lithium 0.1 and vanadium 0.1
^bSalt mix M (mg/kg diet) for maintenance periods: calcium 5,000.0, phosphorus 1,992.0, potassium 3,600.0, sulphur 300.0, sodium 1,019.0, chloride 1,571.0, magnesium 507.0, iron 35.0, zinc 30.0, manganese 10.0, copper 6.0, iodine 0.2, molubdenum 0.15, selenium 0.15, silicon 5.0, chromium 1.0, fluoride 1.0, nickel 0.5, boron 0.5, lithium 0.1, vanadium 0.1
^cVitamin mix (mg/kg diet): retinyl palmitate 2.4, cholecalciferol 0.025, menadione sodium bisulphite 0.8; biotin 0.22, cyanocobalamin 0.01, riboflavin 6.6, thiamin hydrochloride 6.6, tocopherol acetate 100

ISSN: 1880-6562