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Fig. 5 | The Journal of Physiological Sciences

Fig. 5

From: Ethyl 3,4-dihydroxy benzoate, a unique preconditioning agent for alleviating hypoxia-mediated oxidative damage in L6 myoblasts cells

Fig. 5

EDHB supplementation reduce oxidative stress. a Levels of ROS was determined by measuring the fluorescein DCF. An increase in ROS levels was observed in drug groups under hypoxic conditions. b MDA is used as an indicator of lipid peroxidation. MDA levels were estimated by measuring thiobarbituric acid reactive substances. There was decrease in the levels of MDA after hypoxia exposure in EDHB-treated cells. c PO was measured as the carbonyl content after protein derivitization by DNPH. Reduction in PO levels was observed in EDHB-treated cells as compared to controls. Values are mean ± SD. ##Significantly different with respect to normoxia control (p < 0.05), #significantly different with respect to normoxia control (p < 0.01), *significantly different with respect to hypoxia control (p < 0.01), **significantly different with respect to hypoxia control (p < 0.05). NC normoxia control, HC hypoxia control, ND1 normoxia EDHB 500 μM, HD1 hypoxia EDHB 500 μM, ND2 normoxia EDHB 1,000 μM, HD2 hypoxia EDHB 1,000 μM

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