Fig. 4

Effect of EDHB on HIF levels estimated by ELISA. After 0.5 % hypoxic exposure for different times, HIF levels were estimated in cell lysate. A marked increase in the level of HIF was observed in the cells treated with EDHB after hypoxia exposure. Values are mean ± SD. #Significantly different with respect to normoxia control (p < 0.01), ##significantly different with respect to normoxia control (p < 0.05), *significantly different with respect to hypoxia control (p < 0.01), **significantly different with respect to hypoxia control (p < 0.05). NC normoxia control, HC hypoxia control, ND1 normoxia EDHB 500 μM, HD1 hypoxia EDHB 500 μM, ND2 normoxia EDHB 1,000 μM, HD2 hypoxia EDHB 1,000 μM