Fig. 8

Effects of brefeldin A (BFA) on forskolin-stimulated Cl⁻ secretion (a) and conductance (b) in A6 cells treated with or without insulin. After pretreatment with or without 1 μM insulin for 5 h in the culture medium, A6 monolayers were incubated in the medium with or without 5 μg/ml BFA in the presence or absence of insulin for 1 h. After each treatment, A6 monolayers were incubated with 10 μM forskolin under each condition without insulin, and then NPPB-sensitive Isc and Gt were measured by adding NPPB 60 min after application of forskolin. Data are presented as mean ± SEM. n = 4. Ins insulin, NS no significance; *p < 0.05