Fig. 7From: Insulin is involved in transcriptional regulation of NKCC and the CFTR Cl− channel through PI3K activation and ERK inactivation in renal epithelial cellsEffects of PD98059 (PD, a MEK inhibitor, 50 μM) on forskolin-stimulated NPPB-sensitive Isc (a) and Gt (b) in A6 cells treated with or without insulin. After pretreatment with or without 50 μM PD98059 for 1 h in the culture medium, A6 monolayers were incubated in the medium with or without 1 μM insulin for 6 h in the presence or absence of PD98059. After each treatment, 10 μM forskolin was applied, and then NPPB-sensitive Isc and Gt were measured by adding NPPB 60 min after application of forskolin. Data are presented as mean ± SEM. n = 5–8. *p < 0.05Back to article page