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Fig. 3 | The Journal of Physiological Sciences

Fig. 3

From: Insulin is involved in transcriptional regulation of NKCC and the CFTR Cl− channel through PI3K activation and ERK inactivation in renal epithelial cells

Fig. 3

Effects of insulin on phosphorylation of Akt as an indicator of PI3K activity in A6 monolayered cells. a A6 monolayers were treated with 1 μM insulin for the indicated time, and then phosphorylation of Akt (p-Akt) at Ser473 was detected by immunoblotting with an anti-phospho Akt (Ser473)-specific antibody. b Relative amounts of phosphorylated Akt (p-Akt) at Ser473 are shown. Equal loading of cell lysate to each well was confirmed by measuring β-tubulin as an internal control. Data are presented as mean ± SEM. n = 3. *p < 0.05 vs. insulin (−)

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The Journal of Physiological Sciences

ISSN: 1880-6562

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