Fig. 2

Effects of insulin on mRNA expression of NKCC and the CFTR Cl⁻ channel. A6 monolayers were treated with 1 μM insulin for 24 h, and then mRNA expression of NKCC and the CFTR Cl⁻ channel was detected by quantitative real-time PCR. Data are presented as mean ± SEM. n = 5–8. *p < 0.05 vs. insulin (−)