Fig. 1

Time-dependent effect of insulin on forskolin-stimulated NPPB-sensitive Isc (a) and Gt (b) in A6 cells. After pretreating monolayer A6 cells with 1 μM insulin for 0, 1, 3, 6 and 24 h, 10 μM forskolin was applied to the apical and basolateral sites, and then NPPB-sensitive Isc and Gt were detected by the addition of 100 μM NPPB to the apical solution 60 min after application of forskolin. Data are presented as mean ± SEM. n = 5–7. *p < 0.05 vs. control (0 h; without insulin)