Fig. 5
From: Redox proteins are constitutively secreted by skeletal muscle
Mechanism of secretion of TRX-1. a Flow chart of the exosome fractionation of the C2C12 myotube culture medium. b Immunoblotting of the supernatant and exosome fraction. From each fraction, a 50-μl sample was loaded. Alix was used as the exosome marker. TRX-1 was detected in the supernatant. c TRX-1 secreted in the presence of methylamine. The C2C12 myotubes were treated with 1 mM methylamine for 1 h. Each sample was loaded according to the volume-corrected method (Materials and methods). Representative blots showing TRX-1 (C represents control; M represents methylamine treatment) and the calculated graph are presented. Values in the vertical axis are in arbitrary units (AU). Secretion of TRX-1 was unaffected by methylamine treatment (n = 4)