Fig. 3
From: Redox proteins are constitutively secreted by skeletal muscle
Immunoblotting of TRX-1-related proteins and LDH activity in the culture medium of unstimulated (basal) and contracted C2C12 myotubes. The C2C12 myotubes were stimulated by electric pulses of 50 V at 1 Hz for 3 ms at intervals of 997-ms for 6 h. The media collected were concentrated and subjected to immunoblotting and LDH activity measurement. a Representative immunoblots (TRX-1) and calculated graph based on the intensity of the bands. Values in the vertical axis are in arbitrary units (AU). The amount of TRX-1 was significantly higher under this condition (**P < 0.01, n = 4). b LDH activity in medium. LDH activity was significantly higher (*P < 0.05, n = 4). c Representative immunoblots showing peroxiredoxin-1, -3, and -6; glutaredoxin-1, -2, and -3; and myoglobin. Strenuous contraction augmented the abundance of all proteins investigated