Fig. 2

Immunoblotting of the TRX-1 present in the culture supernatant and the LDH activity in unstimulated (basal) and contracted C2C12 myotubes. The C2C12 myotubes were stimulated by electric pulses of a 10 V for 1 h, b 10 V for 3 h, c 30 V for 3 h, or d 12 V for 24 h at 1 Hz for 3 ms duration at intervals of 997 ms. The medium collected was concentrated and was subjected to immunoblotting for TRX-1, and the LDH activity was measured. Left column shows the representative blots and calculated graph based on the intensity of the bands. Values in the vertical axis are in arbitrary units (AU). Right column shows LDH activity under various conditions of stimulation (n = 4 and 5)