Fig. 1
From: Redox proteins are constitutively secreted by skeletal muscle
Immunoblotting of the TRX-1 and related redox proteins present in the C2C12 myotube culture medium. C2C12 myotubes were incubated in fresh serum-free medium for 1, 3, or 6 h. The supernatant from the cultures were then concentrated as described in the “Materials and methods” section and was used for immunoblotting. Each sample was loaded according to the volume-corrected method (Materials and methods). TRX-1 and -2; glutaredoxin-1, -2, and -3; and peroxiredoxin-1, -3, and -6 were detected in the culture medium in a time-dependent manner