Table 1 Summary of representative FRET imaging for MAPKs and related signaling
Target | FRET sensor | Sensor type | Stimulation/application (Cells) | Author | Year | References |
|---|---|---|---|---|---|---|
MEKK3, OSM (scaffold) | – | Intermolecular FRET | Osmostress (Cos-7) | Uhlik et al. | 2003 | [67] |
SAP3Ks | SAP3K reporter | Kinase activity sensor | Osmostress, DNA damage, UV-C, TNF-α (Cos-7, Hela) | Tomida et al. | 2009 | [42] |
Ras, Rap1 | Raichu-Ras, Raichu-Rap1 | Intramolecular FRET | EGF, NGF (Cos-1, PC12) | Mochizuki et al. | 2001 | [33] |
Rac, Cdc42 | Raichu-Rac, Raichu-Cdc42 | Intramolecular FRET | Cell migration (HT1080) | Itoh et al. | 2002 | [34] |
c-Raf | Prin-c-Raf | Intramolecular FRET | EGF (Cos-1) | Terai et al. | 2005 | [68] |
ERK | ERKus | Kinase activity sensor | EGF (MCF-7) | Sato et al. | 2007 | [36] |
ERK | EKAR | Kinase activity sensor | Electrically evoked action potentials (hippocampal neuron) | Harvey et al. | 2008 | [37] |
ERK (C. elegans MPK-1) | ERKy | Kinase activity sensor | In vivo imaging, NaCl-off stimulation (C. elegans sensory neuron) | Tomida et al. | 2012 | [38] |
ERK, JNK | EKAR-EV, JNKAR1-EV | Kinase activity sensor | EGF (Hela) | Komatsu et al. | 2011 | [48] |
ERK | EKAR-EV | Kinase activity sensor | EGF (MCF-10A) | Albeck et al. | 2013 | [104] |
ERK | EKAREV, -NLS | Kinase activity sensor | In vivo imaging, laser ablation (mouse auricular epidermis/dermis) | Kamioka et al. | 2012 | [98] |
ERK | EKAREV-NES, -NLS | Kinase activity sensor | In vivo imaging (mouse mammary gland epithelial cells), fetal bovine serum (MDCK, NRK-52E, Hela, etc.) | Aoki et al. | 2013 | [105] |
JNK (Drosophila) | dJun-FRET | kinase activity sensor | RNAi screening (Drosophila BG-2) | Bakal et al. | 2008 | [39] |
JNK | JNKAR1 | Kinase activity sensor | Anisomycin, osmostress, TNF-α (Hela) | Fosbrink et al. | 2010 | [40] |
JNK | JuCKY | Kinase activity sensor | Anisomycin (Hela) | Suzuki et al. | 2010 | [41] |
Ras | FRas-F | Intermolecular FRET | Electrically evoked action potentials (hippocampal neuron) | Yausda et al. | 2006 | [73] |
Ras | FRas-F | Intermolecular FRET | 2-photon glutamate uncaging (hippocampal neuron) | Harvey et al. | 2008 | [75] |
ERK | EKARnuc | Kinase activity sensor | 2-photon glutamate uncaging (hippocampal neuron) | Zhai et al. | 2013 | [76] |