Fig. 4
Effects of Ca2+ channel blockers on Kv1.3-channel currents and whole-cell membrane capacitance in murine thymocytes. A The effects of 100 μM nifedipine (a) and 10 μM benidipine (b) on Kv1.3-channel currents. Typical whole-cell current traces at different voltage-steps recorded before and after either drug application, and after the washout. The currents were elicited by voltage-steps from the holding potential of −80 to −40, 0, 40, and 80 mV, as depicted in the voltage protocol. Each pulse was applied for a 200-ms duration between 10-s intervals. B Benidipine-induced changes in the thymocyte membrane capacitance, series, and membrane conductance. After establishing the whole-cell configuration, the external solution containing 10 μM benidipine was delivered for 30 s to single thymocytes. Membrane capacitance, series, and membrane conductance were monitored for at least 2 min. N = 5 for each trace. C m membrane capacitance, G s series conductance, G m membrane conductance. Modified from Ref. [43]