Fig. 3

Expression of TRPV2 and TRPV4 in T24 cells. a Amplified fragments of TRPV2, TRPV4, TRPM7, and GAPDH mRNAs in T24 cells. C (control) and M indicate the PCR result using plasmid DNA and markers, respectively. (+) and (−) indicate with and without the RT reaction, respectively. Expected sizes of the amplified TRPV2, TRPM7, TRPV4, and GAPDH fragments are 326, 378, 398, and 742 bp, respectively. b, c Immunocytochemical analysis of T24 cells using anti-TRPV2 antibodies (b) or anti-TRPV4 antibodies (c). Scale bars 200 μm. d, e Changes in cytosolic Ca²⁺ concentrations (indicated by the 340/380 nm excitation ratio, with SEM) in response to a TRPV2 agonist (LPC; D) or a TRPV4 agonist (GSK; E) in T24 cells. f Comparison of LPC-induced increases in cytosolic Ca²⁺ concentrations in the presence and absence of extracellular Ca²⁺, which was normalized to the response of ionomycin. *p < 0.01 vs. Ca²⁺-free. n = 208 and 109 for Ca²⁺ (+) and Ca²⁺-free, respectively. Iono ionomycin