Fig. 1

Expression of TRPV2, TRPV4, and TRPM7 in primary urothelial cells from C3H/HeN mice and in MBT-2 cells. a Amplified fragments of Trpv2, Trpv4, Trpm7, and β-actin mRNAs of primary urothelial cells from C3H/HeN mice and MBT-2 cells. C (control) and M indicate the PCR using plasmid DNA and markers, respectively. (+) and (−) indicate with and without the RT reaction, respectively. Expected sizes for the amplified fragments for Trpv2, Trpm7, Trpv4, and β-actin are 552, 495, 404, and 572 bp, respectively. b Quantitative analysis of mRNA expression of Trpv2, Trpm7, and Trpv4 in C3H/HeN cells and MBT-2 cells using quantitative real-time RT-PCR. *p < 0.05 vs. C3H/HeN cells (n = 3). c, d Phase-contrast images of urothelial cells from C3H/HeN mice (72 h culture, c) and MBT-2 cells (d) under light microscopy. Scale bars 500 μm. e–h Immunocytochemical analysis of urothelial cells from C3H/HeN mice and MBT-2 cells using anti-TRPV2 antibodies (e, f) or anti-TRPV4 antibodies (g, h). Scale bars 200 μm (e, f) and 100 μm (g, h)