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Fig. 5 | The Journal of Physiological Sciences

Fig. 5

From: Electrostatic charge at position 552 affects the activation and permeation of FMRFamide-gated Na+ channels

Fig. 5

Effects of charge alteration at position 552 on the IV relationships of the AkFaNaC. a Steady-state IV relationship of the WT, D552E, D552C, D552N, and D552K. In each figure, the solid curve shows a normalized steady state IV relationship obtained as described in “Materials and methods”. The dashed line is a straight line connecting the reversal potential and the current at −100 mV. b Steady-state IV relationship for the MTSET-modified D552C or the MTSES-modified D552C. The concentrations of MTSET and MTSES were 1 and 10 mM, respectively. IV relationships before MTS treatment are shown by broken lines. Currents were normalized to the current at −100 mV before MTS treatment. c Rectification index (RI) of the WT (n = 6), D552E (n = 3), D552C (n = 25), D552N (n = 6), and D552K (n = 3). The RI was calculated as described in “Materials and methods”. d Modification by MTS reagents of the steady-state IV relationship for the D552C. RI values obtained after treatment with 10 mM MTSES (open circles, n = 4) and 1 mM MTSET (closed circles, n = 9) are shown. The dashed line is the mean RI for the D552C before MTS treatment. e DTT reverses MTS reagent-induced modification of the IV relationship for the D552C. RI values after treatment with 50 mM DTT (squares, n = 4), 1 mM MTSET followed by 50 mM DTT (circles, n = 3), and 10 mM MTSES followed by 50 mM DTT (triangles, n = 3) are shown. The dashed line is the mean RI for the D552C before MTS and/or DTT treatment

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