Fig. 4
FMRFamide dose–response relationships for the D552C before and after treatment with MTS reagents. a Representative FMRFamide-gated currents before and after treatment with 1 mM MTSET. FMRFamide was applied as indicated by a bar. FMRFamide concentration was 0.3, 1, 3, 10, 30, or 100 μM. b Representative FMRFamide-gated currents before and after treatment with 10 mM MTSES. FMRFamide was applied as described above (a). c Dose–response relationships before and after MTSET treatment (n = 4). Smooth lines are best fits to the Hill equation (Eq. 1, “Materials and methods”). EC50 values before and after the treatment were 4.85 ± 1.16 and 2.83 ± 0.17 μM, respectively. Hill coefficients before and after the treatment were 1.08 ± 0.01 and 1.05 ± 0.07, respectively. Maximum response became 103.3 ± 6.6 % of the control after the treatment. d Dose–response relationships before and after MTSES treatment (n = 4). EC50 values before and after the treatment were 4.08 ± 1.35 and 4.63 ± 1.39 μM, respectively. Hill coefficients before and after the treatment were 1.04 ± 0.13 and 1.04 ± 0.06, respectively. Maximum response became 69.0 ± 5.8 % of the control after the treatment