Fig. 2

Effects of MTS reagents on the current amplitude of WT and D552C. a Structures of MTSES and MTSET. A moiety enclosed in a box is appended to cysteine via a disulfide bond. b Representative recording showing effects of MTSET on WT and D552C. FMRFamide-gated currents were elicited by 1 μM FMRFamide for 20 s (bars indicate FMRFamide application). After measuring two consecutive FMRFamide-gated currents with an interval of 10 min, 1 mM MTSET was applied for 2 min (arrow). 10 min later, two consecutive FMRFamide-gated currents were evoked as before. c Representative recording showing effects of 10 mM MTSES on WT and D552C. FMRFamide-gated currents were evoked as described above (b). The steep vertical deflections are the currents in response to ramp commands, which are truncated for graphic reasons