Fig. 2
From: Luminal trypsin induces enteric nerve-mediated anion secretion in the mouse cecum
Ionic basis for the I sc increase induced by 100 μM of mucosal trypsin. Bumetanide (100 μM, serosal) and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB; 100 μM, mucosal) were added, or Cl− was removed (from both the mucosal and serosal bathing solutions) 20 min before adding 100 μM trypsin to the mucosal side. The maximum values for the increase in I sc (ΔI sc) induced by trypsin were determined and are expressed as a percentage of the control response obtained in the adjacent tissue. Each data value is presented as the mean ± SE, with n = 5. **P < 0.01, compared with the control response using the paired t test