Table 1 Antibodies
| Â | Host/label | Dilution | Source |
|---|---|---|---|
Primary antibody | |||
 Anti-EP1 (C-terminal) | Rabbit | 1:400 | Cayman (101740) |
 Anti-EP3 (C-terminal) | Rabbit | 1:400 | Cayman (101760) |
 Anti-α-smooth muscle actin | Mouse | 1:20,000 | Sigma-Aldrich (A5238) |
 Anti-c-Kit | Goat | 1:2,000 | Santa Cruz (SC-1494) |
Secondary antibody | |||
 Donkey anti-rabbit IgG | Alexa594 | 1:400 | Molecular probes (A21207) |
 Goat anti-mouse IgG | Alexa488 | 1:400 | Molecular probes (A11001) |
 Donkey anti-goat IgG | Alexa488 | 1:400 | Molecular probes (A11055) |