Fig. 6

Double-immunostaining for EP₁/EP₃ receptors and c-Kit or neurofilament-200 (NF-200). EP₁/EP₃ and c-Kit IR were visualized using secondary antibodies conjugated with Alexa594 (a, b, red) and Alexa488 (a′, b′, green), respectively. The nuclei were stained with DAPI (blue). c-Kit was used a marker for ICCs. a″, b″ are the merged images of EP₁ with c-Kit and EP₃ with c-Kit, respectively. EP₁/EP₃ and NF-200 IR were visualized using secondary antibodies conjugated with Alexa594 (c, d, red) and Alexa488 (c′, d′, green), respectively. a″, b″ are the merged images of EP₁ with NF-200 and EP₃ with NF-200, respectively. The nuclei were stained with DAPI (blue). NF-200 was used as a marker for neurons. Bars 10 μm. LM longitudinal muscle, CM circular muscle, mg myenteric ganglion