Fig. 5

Immunohistochemistry for the EP₃ receptor in the colonic muscle layer. EP₃ and α-smooth muscle actin IR were visualized using secondary antibodies conjugated with Alexa594 (a, red) and Alexa488 (b, green), respectively. The nuclei were stained with DAPI (blue). α-smooth muscle actin was used a marker for intestinal smooth muscle. c Merged image of a and b. d Magnified image of the longitudinal muscle in c. Arrows in d indicate EP₃ IR around the nuclei of smooth muscle cells. Bars 10 μm. LM longitudinal muscle, CM circular muscle, mg myenteric ganglion (arrowheads)