Fig. 2
Effects of ONO-AE3-248 (EP3 antagonist) on spontaneous GCs. The frequency and amplitude of GCs after the addition of ONO-8713 (EP1 receptor antagonist; 10−7 M), AH6809 (EP1/EP2 antagonist 10−7 M), ONO-AE3-240 (EP3 receptor antagonist; 10−8 and 10−7 M), or ONO-AE3-208 (EP4 antagonist 10−7 M) was measured in the presence of piroxicam. Representative traces of spontaneous GCs in the presence of ONO-8713 (a), GCs in the presence of AH6809 (b), GCs in the presence of ONO-AE3-248 (c), and GCs in the presence of ONO-AE3-208 (d). Effects of ONO-AE3-204 on the frequency (e) and mean amplitude (f) as a percentage of the control values. Values are mean ± SEM (n = 5). ***P < 0.001 and *P < 0.05 (Dunnett’s test). The numbers in each column in (e, f) indicate the number of preparations