Fig. 4
Effects of tachykininergic and cholinergic blockers on contractile responses elicited by high-frequency EFS in the hamster colon. a A representative tracing showing the effects of L-732,138 (1 μM) and SR48968 (1 μM), blockers for tachykinin NK1 and tachykinin NK2 receptors, and atropine (1 μM), an antagonist of muscarinic acetylcholine receptors, on EFS-induced contraction in the hamster colon. EFS was applied using multi-pulses of 20 V each, with a 0.3-ms pulse duration at 20 Hz for 1 s; longitudinal mechanical responses were recorded isometrically. Circles Points of electrical stimulations. b Summary graphs of the effects of L-732,138 (1 μM), SR48968 (1 μM), and atropine (1 μM) on EFS-evoked contraction in the hamster colon (n = 6). Each value represents the mean ± SD. **p < 0.01 and *p < 0.05 compared to the control, which is the response before addition of blockers