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Fig. 2 | The Journal of Physiological Sciences

Fig. 2

From: Hepatocyte growth factor inhibits TGF-β1-induced myofibroblast differentiation in tendon fibroblasts: role of AMPK signaling pathway

Fig. 2

AMPK inhibitor compound C attenuated the inhibitory effect of HGF on the TGF-β1-induced myofibroblastic differentiation in tendon fibroblasts. a Typical cell morphology of tendon fibroblasts under stimulation by TGF-β1 and HGF. be Tendon fibroblasts were treated with TGF-β1 (10 ng/ml), TGF-β1 (10 ng/ml) + HGF (20 ng/ml), or TGF-β1 (10 ng/ml) + HGF (20 ng/ml) + compound C (20 μM) for 48 and 72 h. Then, the mRNA levels of α-SMA (b), Col I α1 (c), Col III (d), and fibronectin (e), four markers of myofibroblastic differentiation, were measure by real-time quantitative PCR analysis; β-actin was used as a housekeeping gene for reference. All data were normalized to β-actin expression (2−ΔΔCt methods). N = 8. *P < 0.05

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