Skip to main content
Fig. 8 | The Journal of Physiological Sciences

Fig. 8

From: Acidification of the synaptic cleft of cone photoreceptor terminal controls the amount of transmitter release, thereby forming the receptive field surround in the vertebrate retina

Fig. 8

Acidification of extracellular surface of cone-driven HCs dissociated from goldfish retinas by high-K+-induced membrane depolarization. A–C A series of time-dependent surface pH (pHs) changes of an HC (pHs reduction: 0.10 pH units from 7.60 during 100 mM K+ application for 3 min). The pHs change is measured by the ratio imaging method (bluish and yellowish colour corresponds to the lower and higher pHs, respectively). D Acidification responses of the HC extracellular surface serially induced by 2 min bath applications of 50 and 13 mM KCl containing Ringers to depolarize the membrane potential. E Acidification of the measured HC extracellular surface is dependent on the KCl concentration. F HC membrane depolarization is dependent on the KCl concentration measured by patch-clamp study in current-clamp mode in comparison with the control Ringer having 2.6 mM KCl. Straight line Nernst equation for K+ shown by \( V = \frac{RT}{F}\log \frac{{[K^{ + } ]_{o} }}{2.6} \). G HC acidification induced by high-K+ is dependent on the membrane depolarization. This graph was composed by combining E and F at 13, 50 and 100 mM KCl (from Jouhou et al. [29])

Back to article page