Fig. 2

Expression of Mest in ADC cells during adipogenic differentiation. a Representative Oil red O staining on day 9 in undifferentiated (undiff.) and differentiated (diff.) ADCs derived from 2 mice (ADCs #1 and ADCs #2). Data were taken from two C57BL/6J mice. b Retention of Oil Red O (a measure of lipid accumulation) in undifferentiated (undiff.) and differentiated (diff.) ADCs on day 9. c PPARγ2 and d Mest gene expression in ADCs during adipocyte differentiation. The levels of gene expression measured in ADCs are the combined results from 4 female pups during adipogenic differentiation; these results were normalized against 36B4 mRNA expression. The ratios of test values to control values were then calculated from measurements taken on day 0 for Mest and day 3 for PPARγ2, and expressed as mean ± SD (n = 4). ND = not detected. Single and double asterisks represent significance in the test values compared to control values measured on day 0 (P < 0.05 and P < 0.01 respectively). e Representative RT-PCR results for Mest and 36B4 expression in ADCs