Fig. 4

Effects of mitoK_Ca and mitoK_Ca channel openers on mitochondrial membrane potential, ROS production, cytosolic Ca²⁺ concentration and cell length. a Representative sequential confocal images of TMRE (red)/DCFH (yellow)-loaded and TMRE/Fluo-4 (green)-loaded myocytes before and after 15 min of intermittent laser illumination. b Changes in ROS production (DCFH fluorescence). c Changes in mitochondrial membrane potential (TMRE fluorescence). d Changes in the cytosolic Ca²⁺ concentration (Fluo-4 fluorescence). e Changes in cell length (final/initial of cell length). N before laser illumination, CT control, Dia diazoxide, n number of cells. *P < 0.05 versus CT (color figure online)