Fig. 1

Change in 5-CMF fluorescence intensity by NEM. a NEM-induced shift of 5-CMF fluorescence histogram. 5-CMF fluorescence was measured from cells that were not stained with propidium iodide. Each histogram was constructed with 2,500 cells. b Concentration-dependent attenuation of 5-CMF fluorescence by NEM. Columns and bars indicate means and standard deviations, respectively, of five experiments. ^# P < 0.05, ^## P < 0.01 significant differences compared with control