Fig. 2

The localization of GFP-tagged wild-type hERG (a) and G601S (b) the day after transfection of their respective expression constructs. The left-hand panels show confocal-imaged, GFP-labeled G601S and WT hERG channels in HEK293 cells. The right-hand panels show merged Nomarski images and GFP fluorescence images. At 24 h after transfection, the protein localizations of WT hERG and G601S are indistinguishable. On day 2, approximately 48 h after transfection, WT hERG (c) was localized at the plasma membrane more than in the cytosol (arrow). In contrast, G601S was not trafficked through the cytosol to the plasma membrane (d) (arrow). All scale bars are 5 μm