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Fig. 3 | The Journal of Physiological Sciences

Fig. 3

From: ATP hydrolysis-dependent asymmetry of the conformation of CFTR channel pore

Fig. 3

Effects of polymers on the single-channel conductance of the CFTR Cl− channel in the absence of AMP-PNP. a Relative changes in the unitary single-channel conductance (left axis) and bulk conductivity (right axis) as a function of the hydrodynamic radius (R h) of PEG molecules (R h = 0.45, 0.53, 0.62, 0.75, 0.95, 1.16, 1.39 and 1.84 nm for PEG 200, 300, 400, 600, 1,000, 1,540, 2,000 and 3,400, respectively). Filled circles represent the inward slope conductance obtained in the inside-out mode with polymers added to the bath solution (configuration 3). Open circles and open triangles represent the outward slope conductance obtained with polymers added to the pipette solution in the inside-out mode (configuration 1) and the cell-attached mode (configuration 2), respectively. Data were collected from 5–9 different patches for each polymer. Open squares represent the relative decrease in the bulk conductivity of the solutions used in these experiments (n = 5). *Significantly different from the slope conductance obtained in control conditions without polymers at P < 0.05. #Significantly different from the respective slope conductance obtained with polymers added to the pipette solution in the inside-out mode at P < 0.05. Inset Schematic illustrations of configurations 1–3 used in these experiments. b Filling coefficients calculated according to Eq. 1 as a function of the hydrodynamic radius of PEG molecules. Symbols are the same as in (a). Solid lines are linear fits to the descending parts of the curves with correlation coefficient of −0.90 and −0.99 for extracellular and intracellular PEG application experiments, respectively. A horizontal dashed line corresponds to zero filling. R1 and R2 denote the radius of the extracellular and intracellular entrance of the CFTR Cl− channel pore, respectively

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